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BIOSENSOR EXPLANATION
Principles and Construction The Axiom Biosensor is based on frequency shifts due to differences
in acoustic impedance. As a simple illustration of this concept, consider the cups
in the images below.
 
The sounds that the cup makes when struck has a
particular frequency which is determined by its acoustical impedance. With the soft
material attached to the cup, the cup's acoustical impedance has been altered, and
therefore its frequency shifts.
In the Axiom Biosensor systems, a piezoelectric transducer (PZT) element acts
as the cup, and when there is an electric input, it vibrates at its own inherent resonance
frequency. When it contacts some material, such as soft tissue, this frequency
shifts. The amount of shift in frequency is determined by the material's acoustical
impendance, and extensive research has shown that it is directly correlated (R2
= 0.95 - 0.98) with the hardness/softness of the material. The change in frequency,
or Df, is defined
as the difference between the new frequency, fx, and the initial frequency, f0,
shown as Df = fx
- f0.
The basic design of the Pen-Type tactile sensor
system, Axiom's first Biosensor, can be seen in the image below. Axiom
produces several different designs of Biosensors for numerous applications. Smaller
probes may be equipped with only a tactile sensor for simplification, while the more
elaborate Venustron System also has pressure and movement
sensors which make it especially effective for measuring visco-elasticity such as that of
skin. Most of our systems, excluding custom-made ones, can be seen on the Sensor Systems page. |
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Mathematical
Basis
A more thorough explanation of the Biosensor,
including its mathematical basis, follows. The sensor consists of a piezoelectric
transducer made of ceramics such as lead zirconate titanate (PZT) and a vibration pickup
(made of PZT or ployvinylidene fluoride (PVF2) film). When an alternating voltage is
applied across its electrode, as is well known, the PZT element is able to vibrate freely
in the direction of its length.
The
pickup detects a vibration that is generated in the rectangular PZT element. As the
amplification is increased in this system, and since the pickup transducer detects this
frequency and feeds a small alternating signal to a driving amplifier, the feedback
circuit system oscillates at the resonance frequency. Hence, the driving amplifier always
drives the PZT element at its resonance frequency. If the free end of the PZT element is
pressed against a surface, as shown in this figure, the resonance frequency of the
feedback system changes. This depends on the acoustic impedance of the object.
Such basic behavior of the Biosensor system can be
explained and approximated in terms of the vibration mode of a finite rod as follows:
If the end of a finite rod is closed by the unknown
impedance Zx, at position l on its length, in general, the
theoretical treatment of a vibrating rod is well known. After considering the resonance
frequency for the loaded and unloaded conditions in the feedback system, the change in
resonance frequency may be written as:
 |
(1) |
where V0 is an equivalent
velocity and Z0 an equivalent impedance of the sensor system. b is the reactance of the
impedance of the unknown object, Zx, which may be expressed in the form:
 |
(2) |
where a is a resistance. The reactance, b, may be adequately written [5] as:
 |
(3) |
and
 |
(4) |
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|
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(5) |
where r = density, n = Poisson's ratio, E = Young's modulus, S = pr2 and r
= radius of contact area. mx is the inertia term and Cx
the surface compliance, so the stiffness, kx = 1/Cx.
As is shown in eqns. (4) and (5), the inertia term, mx, can be expressed
as the third power of the radius r, and the stiffness, kx, as the
first power of r. If the contact area of the tactile sensor is r < 1.0,
the stiffness term, kx, will be larger than the inertia term, mx.
On the other hand, at r > 1.0, the stiffness term, kx, may be
neglected. Then the change in resonance frequency caused by the stiffness loading effect
may be written as:
 |
(6) |
and for the mass loading,
 |
(7) |
where k0 is an equivalent
stiffness and m0 an equivalent mass for an equivalent impedance of the
sensor system.
In the above-mentioned principles, there will be
losses occurring in the specimens that will contribute to the resistance, a. For example, wave
attenuations take place within the medium and at the boundaries. However, such effects on
the change in resonance frequency can be approximately included in the unknown impedance, Zx.
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After starting a sample measurement on the
computer, the user applies the Biosensor by gently pressing straight down
(perpendicularly) on the sample for a few seconds.
As shown in the figure, the electronic signals to
and from the tactile sensor pass through an amplifier and filter before proceeding to a
frequency counter where they are converted into digital signals. These signals are then
sent to a central processing unit (CPU) where they are sequentially processed after which
they are displayed on a computer monitor in "real time" and stored on the
computer's hard disk. The recorded data are written in text format and are therefore easy
to graphically display with analysis software.
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Real time graphs run across the computer screen as the
Biosensor is applied to the sample which appear similar to the ones in the diagram. |
Data Interpretation
The tactile reading is expressed in Hz and is given
as Df. As the
tactile curve dips lower, this indicates a softer material. Hence, the softer the
material, the more negative Df becomes. A less negative or a positive Df indicates a harder or stiffer substance.
The user can observe how the different amounts of
pressure lead to corresponding changes in the tactile readings. Usually, a saturation
point is quickly achieved, where increasing amounts of pressure do not significantly
affect the tactile values. This is, of course, quite similar to the human sense of touch
when used to gauge the stiffness of material. A particular value for stiffness, usually
averaged from around the saturation point, can then be selected from the graph via the
analysis software.
The Biosensor is generally used to determine
stiffness values for comparative research; for example, the differences in softness among
materials, or the location of hard substances within materials. As such, stiffness values
in terms of Hz can be used for comparison and statistical analysis.
Due to its motor control, the Venustron System provides more data as
it measures material while both pushing down on it and retracting thereby generating
hysteresis curves. These curves can be used to gain an understanding of the
material's elastic properties.
Please see sample data and graphs from various
research for a more in-depth understanding of the Biosensor's abilities.
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