Axiom Co., Ltd. HOW SOFT?  The Axiom Biosensor Knows!


BIOSENSOR EXPLANATION

Principles and Construction 

The Axiom Biosensor is based on frequency shifts due to differences in acoustic impedance.  As a simple illustration of this concept, consider the cups in the images below.

Dingggg goes the cup...Dongggg goes the cup...

The sounds that the cup makes when struck has a particular frequency which is determined by its acoustical impedance.  With the soft material attached to the cup, the cup's acoustical impedance has been altered, and therefore its frequency shifts.

PZT DiagramIn the Axiom Biosensor systems, a piezoelectric transducer (PZT) element acts as the cup, and when there is an electric input, it vibrates at its own inherent resonance frequency.  When it contacts some material, such as soft tissue, this frequency shifts.  The amount of shift in frequency is determined by the material's acoustical impendance, and extensive research has shown that it is directly correlated (R2 = 0.95 - 0.98) with the hardness/softness of the material.  The change in frequency, or Df, is defined as the difference between the new frequency, fx, and the initial frequency, f0, shown as Df = fx - f0.

The basic design of the Pen-Type tactile sensor system, Axiom's first Biosensor, can be seen in the image below.Click here for details about the Biosensor SystemsAxiom produces several different designs of Biosensors for numerous applications.  Smaller probes may be equipped with only a tactile sensor for simplification, while the more elaborate Venustron System also has pressure and movement sensors which make it especially effective for measuring visco-elasticity such as that of skin.  Most of our systems, excluding custom-made ones, can be seen on the Sensor Systems page.

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Mathematical Basis

A more thorough explanation of the Biosensor, including its mathematical basis, follows. The sensor consists of a piezoelectric transducer made of ceramics such as lead zirconate titanate (PZT) and a vibration pickup (made of PZT or ployvinylidene fluoride (PVF2) film). When an alternating voltage is applied across its electrode, as is well known, the PZT element is able to vibrate freely in the direction of its length.

Principle of BiosensorThe pickup detects a vibration that is generated in the rectangular PZT element. As the amplification is increased in this system, and since the pickup transducer detects this frequency and feeds a small alternating signal to a driving amplifier, the feedback circuit system oscillates at the resonance frequency. Hence, the driving amplifier always drives the PZT element at its resonance frequency. If the free end of the PZT element is pressed against a surface, as shown in this figure, the resonance frequency of the feedback system changes. This depends on the acoustic impedance of the object.

Such basic behavior of the Biosensor system can be explained and approximated in terms of the vibration mode of a finite rod as follows:

If the end of a finite rod is closed by the unknown impedance Zx, at position l on its length, in general, the theoretical treatment of a vibrating rod is well known. After considering the resonance frequency for the loaded and unloaded conditions in the feedback system, the change in resonance frequency may be written as:

Formula 1 (1)

where V0 is an equivalent velocity and Z0 an equivalent impedance of the sensor system. b is the reactance of the impedance of the unknown object, Zx, which may be expressed in the form:

Formula 2 (2)

where a is a resistance. The reactance, b, may be adequately written [5] as:

Formula 3 (3)

and

Formula 4 (4)
Formula 5 (5)

where r = density, n = Poisson's ratio, E = Young's modulus, S = pr2 and r = radius of contact area. mx is the inertia term and Cx the surface compliance, so the stiffness, kx = 1/Cx. As is shown in eqns. (4) and (5), the inertia term, mx, can be expressed as the third power of the radius r, and the stiffness, kx, as the first power of r. If the contact area of the tactile sensor is r < 1.0, the stiffness term, kx, will be larger than the inertia term, mx. On the other hand, at r > 1.0, the stiffness term, kx, may be neglected. Then the change in resonance frequency caused by the stiffness loading effect may be written as:

Formula 6 (6)

and for the mass loading,

Formula 7 (7)

where k0 is an equivalent stiffness and m0 an equivalent mass for an equivalent impedance of the sensor system.

In the above-mentioned principles, there will be losses occurring in the specimens that will contribute to the resistance, a. For example, wave attenuations take place within the medium and at the boundaries. However, such effects on the change in resonance frequency can be approximately included in the unknown impedance, Zx.

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Biosensor SystemApplication and Data Display

After starting a sample measurement on the computer, the user applies the Biosensor by gently pressing straight down (perpendicularly) on the sample for a few seconds.

As shown in the figure, the electronic signals to and from the tactile sensor pass through an amplifier and filter before proceeding to a frequency counter where they are converted into digital signals. These signals are then sent to a central processing unit (CPU) where they are sequentially processed after which they are displayed on a computer monitor in "real time" and stored on the computer's hard disk. The recorded data are written in text format and are therefore easy to graphically display with analysis software.

 

Biosensor graphReal time graphs run across the computer screen as the Biosensor is applied to the sample which appear similar to the ones in the diagram.

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Data Interpretation

The tactile reading is expressed in Hz and is given as Df. As the tactile curve dips lower, this indicates a softer material. Hence, the softer the material, the more negative Df becomes. A less negative or a positive Df indicates a harder or stiffer substance.

The user can observe how the different amounts of pressure lead to corresponding changes in the tactile readings. Usually, a saturation point is quickly achieved, where increasing amounts of pressure do not significantly affect the tactile values. This is, of course, quite similar to the human sense of touch when used to gauge the stiffness of material. A particular value for stiffness, usually averaged from around the saturation point, can then be selected from the graph via the analysis software.

The Biosensor is generally used to determine stiffness values for comparative research; for example, the differences in softness among materials, or the location of hard substances within materials. As such, stiffness values in terms of Hz can be used for comparison and statistical analysis.

Due to its motor control, the Venustron System provides more data as it measures material while both pushing down on it and retracting thereby generating hysteresis curves.  These curves can be used to gain an understanding of the material's elastic properties.

Please see sample data and graphs from various research for a more in-depth understanding of the Biosensor's abilities.

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